Plasmid

Part:BBa_K4841019:Design

Designed by: Zhang Huixin   Group: iGEM23_TSBC-SZ   (2023-08-28)


pACYC-pJ23117-esaR(I70V)-rrnBT -LSPT-esaI-BCD22-P8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 583
    Illegal NheI site found at 1421
    Illegal NheI site found at 1444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1471
    Illegal BamHI site found at 2492
    Illegal XhoI site found at 2565
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2238
    Illegal NgoMIV site found at 2823
    Illegal NgoMIV site found at 2983
    Illegal NgoMIV site found at 3337
    Illegal NgoMIV site found at 3917
    Illegal NgoMIV site found at 4902
    Illegal NgoMIV site found at 5157
    Illegal AgeI site found at 669
    Illegal AgeI site found at 993
    Illegal AgeI site found at 2089
    Illegal AgeI site found at 3741
    Illegal AgeI site found at 4201
    Illegal AgeI site found at 4850
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NO


Source

NO

References